Composite

Part:BBa_K4759217:Design

Designed by: Jianhua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS1-Fdr-0978-RBS2-Fdx-1499-linker-GFP1-10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1306
    Illegal PstI site found at 368
    Illegal PstI site found at 694
    Illegal PstI site found at 1009
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1306
    Illegal PstI site found at 368
    Illegal PstI site found at 694
    Illegal PstI site found at 1009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1306
    Illegal BglII site found at 1124
    Illegal BglII site found at 2295
    Illegal BamHI site found at 1300
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1306
    Illegal PstI site found at 368
    Illegal PstI site found at 694
    Illegal PstI site found at 1009
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1306
    Illegal PstI site found at 368
    Illegal PstI site found at 694
    Illegal PstI site found at 1009
    Illegal NgoMIV site found at 712
    Illegal AgeI site found at 784
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1429


Design Notes

Screening the optimal redox partners for OleP by bimolecular fluorescence complementation technology (BiFC)

Source

References